Partic. vol. 24 pp. 170-176 (February 2016)
doi: 10.1016/j.partic.2014.12.015

Gas-assisted magnetic separation for the purification of proteins in batch systems

Zhini Liu^a,b, Liangrong Yang^b,*, Tingting Dong^b, Wensong Li^a,b, Xitong Sun^b, Menghao Zhu^b, Zhengkang Duan^a, Qingfen Liu^b, Huizhou Liu^b,*

lryang@home.ipe.ac.cnhzliu@home.ipe.ac.cn

Abstract

In this paper, gas-assisted magnetic separation (GAMS), a technique that combines magnetic separation with flotation, was investigated for the potential large-scale separation of proteins. The GAMS process includes adsorption of target proteins and magnetic separation to recover protein-loaded magnetic particles from the dilute biosuspension with the assistance of bubbles. Microsized ethylenediamine-functionalized poly(glycidyl methacrylate) superparamagnetic microspheres (MPMs) and bovine serum albumin (BSA) were used as a model system. The feasibility of GAMS for capturing BSA-loaded MPMs from an appropriate medium was shown. High recovery of BSA-loaded MPMs was obtained by simple adjustment of the initial solution pH without extra detergents and antifoaming agents. The GAMS conditions were consistent with the adsorption conditions, and no proteins were desorbed from the MPMs during this process. Under the optimal conditions, the separation rate and recovery percentage reached 410 mL/min and 98% in 0.61 min, respectively. Conformational changes of BSA during the GAMS process were investigated by fluorescence spectroscopy and circular dichroism spectrometry.

Graphical abstract

Keywords

Adsorption; Magnetic separation; Flotation; Recovery; BSA conformation